Blocking Assay:Article Title: Mechanisms of amyloid-β34 generation indicate a pivotal role for BACE1 in amyloid homeostasis.
Article Snippet: .. 250 μl Stabil Coat®Immunoassay Stabilizer (SurModics Inc.) was used for blocking and plates were incubated for 2 h at room temperature with gentle shaking. .. 50 μl of 0.075 μg/ml detection antibody, W02-biotin, in assay buffer (90% 11 mM NaH2PO4, 85 mM Na2HPO4, 137 mM NaCl, (pH 7.4), 0.5% Tween-20, 1.5% BSA, 0.01% Thimerosal, and 10% SeaBlock blocking buffer (Thermo Fisher Scientific Inc.) was loaded to the wells together with 50 μl sample (cell media) or calibrator (synthetic peptide standards diluted in DMEM or DMEM/F12).
Article Title: Mechanisms of amyloid-β34 generation indicate a pivotal role for BACE1 in amyloid homeostasis
Article Snippet: .. 250 μl Stabil Coat®Immunoassay Stabilizer (SurModics Inc.) was used for blocking and plates were incubated for 2 h at room temperature with gentle shaking. .. 50 μl of 0.075 μg/ml detection antibody, W02-biotin, in assay buffer (90% 11 mM NaH 2 PO 4 , 85 mM Na 2 HPO 4 , 137 mM NaCl, (pH 7.4), 0.5% Tween-20, 1.5% BSA, 0.01% Thimerosal, and 10% SeaBlock blocking buffer (Thermo Fisher Scientific Inc.) was loaded to the wells together with 50 μl sample (cell media) or calibrator (synthetic peptide standards diluted in DMEM or DMEM/F12).
Incubation:Article Title: Mechanisms of amyloid-β34 generation indicate a pivotal role for BACE1 in amyloid homeostasis.
Article Snippet: .. 250 μl Stabil Coat®Immunoassay Stabilizer (SurModics Inc.) was used for blocking and plates were incubated for 2 h at room temperature with gentle shaking. .. 50 μl of 0.075 μg/ml detection antibody, W02-biotin, in assay buffer (90% 11 mM NaH2PO4, 85 mM Na2HPO4, 137 mM NaCl, (pH 7.4), 0.5% Tween-20, 1.5% BSA, 0.01% Thimerosal, and 10% SeaBlock blocking buffer (Thermo Fisher Scientific Inc.) was loaded to the wells together with 50 μl sample (cell media) or calibrator (synthetic peptide standards diluted in DMEM or DMEM/F12).
Article Title: Mechanisms of amyloid-β34 generation indicate a pivotal role for BACE1 in amyloid homeostasis
Article Snippet: .. 250 μl Stabil Coat®Immunoassay Stabilizer (SurModics Inc.) was used for blocking and plates were incubated for 2 h at room temperature with gentle shaking. .. 50 μl of 0.075 μg/ml detection antibody, W02-biotin, in assay buffer (90% 11 mM NaH 2 PO 4 , 85 mM Na 2 HPO 4 , 137 mM NaCl, (pH 7.4), 0.5% Tween-20, 1.5% BSA, 0.01% Thimerosal, and 10% SeaBlock blocking buffer (Thermo Fisher Scientific Inc.) was loaded to the wells together with 50 μl sample (cell media) or calibrator (synthetic peptide standards diluted in DMEM or DMEM/F12).
Gentle:Article Title: Mechanisms of amyloid-β34 generation indicate a pivotal role for BACE1 in amyloid homeostasis.
Article Snippet: .. 250 μl Stabil Coat®Immunoassay Stabilizer (SurModics Inc.) was used for blocking and plates were incubated for 2 h at room temperature with gentle shaking. .. 50 μl of 0.075 μg/ml detection antibody, W02-biotin, in assay buffer (90% 11 mM NaH2PO4, 85 mM Na2HPO4, 137 mM NaCl, (pH 7.4), 0.5% Tween-20, 1.5% BSA, 0.01% Thimerosal, and 10% SeaBlock blocking buffer (Thermo Fisher Scientific Inc.) was loaded to the wells together with 50 μl sample (cell media) or calibrator (synthetic peptide standards diluted in DMEM or DMEM/F12).
Article Title: Mechanisms of amyloid-β34 generation indicate a pivotal role for BACE1 in amyloid homeostasis
Article Snippet: .. 250 μl Stabil Coat®Immunoassay Stabilizer (SurModics Inc.) was used for blocking and plates were incubated for 2 h at room temperature with gentle shaking. .. 50 μl of 0.075 μg/ml detection antibody, W02-biotin, in assay buffer (90% 11 mM NaH 2 PO 4 , 85 mM Na 2 HPO 4 , 137 mM NaCl, (pH 7.4), 0.5% Tween-20, 1.5% BSA, 0.01% Thimerosal, and 10% SeaBlock blocking buffer (Thermo Fisher Scientific Inc.) was loaded to the wells together with 50 μl sample (cell media) or calibrator (synthetic peptide standards diluted in DMEM or DMEM/F12).
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